Journal: Journal of Inflammation Research

Article Title: Lycopus lucidus Turcz Exerts Neuroprotective Effects Against H 2 O 2 -Induced Neuroinflammation by Inhibiting NLRP3 Inflammasome Activation in Cortical Neurons

doi: 10.2147/JIR.S305031

Figure Lengend Snippet: LLT protects cortical neurons against H 2 O 2 -induced neuronal damage. ( A ) Schematic depiction of experimental design for primary cortical neurons ( B ) Cell viability assay results of cortical neurons treated with various concentrations of LLT without H 2 O 2 induction at 24 h, n = 3. ( C ) CCK-8 results of H 2 O 2 -treated cortical neurons treated with various concentrations of LLT at 24 h, n = 3. ( D ) The total number of live and dead cells calculated from cellular fluorescence images of each group. n = 10. ( E ) The percentage of apoptotic cells analyzed by flow cytometry of Annexin-V-FITC/PI-PE double staining. n = 5. ( F ) Fluorescence images of live and dead (red) cells for analysis of the neuroprotective effect of LLT at different concentrations on H 2 O 2 -treated cortical neurons. Scale bar = 200 µm. ( G ) Representative flow cytometric plot using Annexin-V/PI to confirm the mode of cell death. Blank = non-treatment, H 2 O 2 = H 2 O 2 only, LLT = H 2 O 2 +LLT extract. Significant differences are indicated as follows and were analyzed via one-way ANOVA with Tukey’s post-hoc test: # p < 0.001 vs the blank group; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs the H 2 O 2 group.

Article Snippet: Apoptotic cell death was detected using an Annexin V-Fluorescein isothiocyanate (FITC) propidium iodide (PI)-phycoerythrin apoptosis detection kit (Abcam) as described previously.

Techniques: Viability Assay, CCK-8 Assay, Fluorescence, Flow Cytometry, Double Staining

Journal: Theranostics

Article Title: Role of damaged mitochondrial transfer in alpha-particle generator 212 Pb radiation-induced bystander effect

doi: 10.7150/thno.101922

Figure Lengend Snippet: Radiation-induced bystander effect triggered by alpha-particle 212 Bi. A) Decay scheme of alpha-particle generator 212 Pb. B) Schematic representation of the experimental design for the subsequent cytotoxic effect study. C, D, and E) The cytotoxic effect of 212 Bi with gradient concentration on donor cells for 6 h(C), 12h (D), 24 h (E), respectively (n=5). F, G, and H) The cytotoxic effect of supernatant from donor cells after 6 h (F), 12 h (G), and 24 h (H) 212 Bi-irradiation with gradient concentration on recipient cells for 24 h (n=5). I) Flow cytometry patterns of recipient cells and donor cells stained with annexin Ⅴ-FITC and propidium iodide (PI) for determining the apoptosis level. J) The cytotoxic effect of supernatant after 24 h 212 Bi-irradiation with gradient concentration on rotenone-pretreated recipient cells for 24 h.

Article Snippet: Calcein/Propidium Iodide (PI) cell viability/cytotoxicity assay kit, reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG) assay kits, mitochondrial membrane potential assay kit with JC-1, and the Annexin V-Fluorescein isothiocyanate (FITC) apoptosis assay kit were obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Concentration Assay, Irradiation, Flow Cytometry, Staining

Journal: Theranostics

Article Title: Role of damaged mitochondrial transfer in alpha-particle generator 212 Pb radiation-induced bystander effect

doi: 10.7150/thno.101922

Figure Lengend Snippet: The transfer of damaged mitochondria results in alpha-particle radiation-induced bystander effect. A) Schematic representation of the experimental design for the subsequent study. B, C, and D) The cytotoxic effect of various treatments with gradient concentration on recipient cells for 24 h was evaluated by CCK-8 assays (n=5 per group). E and F) Flow cytometry patterns of recipient cells stained with annexin Ⅴ-FITC and PI after various treatments for determining the apoptosis level. G) Cytotoxicity of various treatments on recipient cells by using co-staining with PI and Calcein-AM. H) Visualization of JC-1 monomer (Green) and JC-1 aggregate (Red) in recipient cells after various treatments.

Article Snippet: Calcein/Propidium Iodide (PI) cell viability/cytotoxicity assay kit, reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG) assay kits, mitochondrial membrane potential assay kit with JC-1, and the Annexin V-Fluorescein isothiocyanate (FITC) apoptosis assay kit were obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Concentration Assay, CCK-8 Assay, Flow Cytometry, Staining

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Paeoniflorin inhibits cell growth and induces cell cycle arrest through inhibition of FoxM1 in colorectal cancer cells.

doi: 10.1080/15384101.2017.1407892

Figure Lengend Snippet: Figure 1. PF inhibited cell proliferation and induced apoptosis and cell cycle arrest. A. Colon cancer cell viability was detected after PF treatment for 24 h, 48 h and 72 h, respectively, using MTT assay. P < 0.05, P < 0.01, compared to the control groups. B. Cell cycle analysis was performed by Flow cytometry in HCT116 cells treated with PF. C. PF-induced colon cancer cell apoptosis was accessed by Flow cytometry.

Article Snippet: Annexin apoptosis assay kit was purchased from Beyotime Biotechnology (Shanghai, China).

Techniques: MTT Assay, Control, Cell Cycle Assay, Flow Cytometry

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Paeoniflorin inhibits cell growth and induces cell cycle arrest through inhibition of FoxM1 in colorectal cancer cells.

doi: 10.1080/15384101.2017.1407892

Figure Lengend Snippet: Figure 3. Overexpression of FoxM1 abrogated PF-induced cell growth inhibition and apoptosis. A. Left panel: The expression of FoxM1 was detected by Western blotting analysis in HCT116 cells after FoxM1 overexpression plus PF treatment. Right panel; Quantitative results were illustrated for left panel. CTR: control; PF: Paeoniflorin; PF+cDNA: Paeoniflorin plus FoxM1 cDNA. B. Cell growth was measured by MTT assay after FoxM1 overexpression in combination with PF treatment. C. Cell cycle was accessed by Flow cytometry after FoxM1 overexpression in combination with PF treatment. D. Apoptotic cells were accessed by Flow cytometry after after FoxM1 overexpression in combination with PF treatment.

Article Snippet: Annexin apoptosis assay kit was purchased from Beyotime Biotechnology (Shanghai, China).

Techniques: Over Expression, Inhibition, Expressing, Western Blot, Control, MTT Assay, Flow Cytometry

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Paeoniflorin inhibits cell growth and induces cell cycle arrest through inhibition of FoxM1 in colorectal cancer cells.

doi: 10.1080/15384101.2017.1407892

Figure Lengend Snippet: Figure 5. Knockdown of FoxM1 enhanced PF-mediated cell proliferation inhibition and apoptosis. A. The expression of FoxM1 was detected by Western blotting analysis in HCT116 cells after FoxM1 down-regulation plus PF treatment. Right panel: Quantitative results were illustrated for left panel. CTR: Control. PF: Paeoniflorin; PF+siRNA: Paeoniflorin plus FoxM1 siRNA. P < 0.05, vs control; # P < 0.05 vs FoxM1 siRNA transfection. B. MTT assay was performed to detect the effect of FoxM1 down-regulation in combination with PF treatment on HCT116 cell growth. P < 0.05, vs control; # P < 0.05 vs FoxM1 siRNA transfection. C. Cell cycle was accessed by Flow cytometry after FoxM1 downregulation in combination with PF treatment. D. Apoptotic cells were detected by Flow cytometry in HCT116 cells after FoxM1 siRNA transfection and PF treatment.

Article Snippet: Annexin apoptosis assay kit was purchased from Beyotime Biotechnology (Shanghai, China).

Techniques: Knockdown, Inhibition, Expressing, Western Blot, Control, Transfection, MTT Assay, Flow Cytometry

Journal: International Journal of Oncology

Article Title: Eupalinolide A induces autophagy via the ROS/ERK signaling pathway in hepatocellular carcinoma cells in vitro and in vivo

doi: 10.3892/ijo.2022.5421

Figure Lengend Snippet: Effect of EA on apoptosis and necroptosis of human hepatocellular carcinoma cells. (A and B) Western blot analysis of the expression levels of apoptosis and necroptosis-related proteins at 48 h in MHCC97-L and HCCLM3 cells. β-actin was used as a control. (C and D) Effect of EA on apoptosis in MHCC97-L and HCCLM3 cells, as determined by flow cytometry. n=5. (E) Viability of MHCC97-L and HCCLM3 cells treated with or without apoptosis or necroptosis inhibitors was measured by Cell Counting Kit 8 assays. n=6. All data are shown as the mean ± SD. *** P<0.001. EA, eupalinolide A; C-, cleaved; p-, phosphorylated.

Article Snippet: The cells were then collected, washed with cold PBS and suspended in 200 μ l binding buffer (Annexin V-FITC/PI apoptosis assay kit; Neobioscience Technology Co., Ltd.) at a density of 5×10 5 /ml.

Techniques: Western Blot, Expressing, Control, Flow Cytometry, Cell Counting